Kohana, daughter of Ketsurui Ayame, was a young princess who was (according to official reports) kidnapped, raped, and murdered by Kenji Ashigari after Kenji found his lover Ayame had fallen in love with Kitsurugi Uesu. The secret reality is that Kenji was actually set up by Ketsurui Kikyo, who murdered him along with Kohana to eliminate Uesu's competition.